The cuticle of insects has several functions, such as protecting against environmental conditions, pathogens, and other insects, as well as supporting the body. The epicuticle is the external layer of the cuticle and consists of two layers: the first one, composed of chitin, and the second one, composed of hydrocarbons, which helps the insect avoid dehy-dration and damage by ultraviolet (UV) rays (Hadley 1984; Vrkoslav et al. 2010). These hydrocarbons are inter- and intraspecific recognition signals because they vary in species, age, sex, and physiological stage. Nevertheless, cuticular hydrocarbons (CHC) have been studied to taxonomically identify pest insects, such as Ceratitis capitata,Ceratitis anonae, Ceratitis fasciventris Bezzi, Ceratitis rosa, and Anastrepha fraterculus (Vaníčková et al. 2014). However, our interest focuses on inter- and intraspecific recognition (Blomquist 2010). In this work, we studied the CHC profile of virgin males and females of differ-ent ages of Anastrepha curvicauda, formerly Toxotrypana (Diptera: Tephritidae) (Norrbom et al. 2018)