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E-book Sugar Beet : A competitive innovation
After cutting into small fragments of their DNA, millions of reads of 100 bases in length and in both directions of reading (forward and reverse) were performed for each of the sixteen plants, also called accessions. Thus, for each accession, a library of short sequences of its genome was obtained. We could compare them to bags containing the pieces of a puzzle that must be assembled to obtain the DNA sequence of these accessions. The background image of the puzzle which makes it possible to order them is a reference sequence which had previously been obtained in a project originating from the SESVanderhave company, a subsidiary of the Florimond Desprez group, and the German programme Gabi. So it was a question of starting from this reference sequence and aligning on it the readings of the elite and the sixteen accessions. We then identified differences between the sequence of the elite and that of the different accessions aligned with the reference and defined molecular markers between the elite and the accessions.
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